Vitamin B6 - Pyridoxine

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Figure 1. Pyridoxine, C₈H₁₁NO₃, MW=169,18 g/mol
 
Vitamin B₆ occurs in foods as pyridoxine (PN), pyridoxamine (PM), pyridoxal (PL) and the corresponding phosphate esters (PNP, PMP and PLP). Also glycosylated forms may be present in foods.  
 
Synonyms for vitamin B₆:

• pyridoxine, adermin

 
Summary of the determination by HPLC

Summary of the determination by microbiological assay

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Golden standards

There are two European Standards for the determination of vitamin B6. Methods are described in child pages where you can get by clicking the links below:

EN 14164:2008 Foodstuffs - Determination of vitamin B6 by HPLC

EN 14166:2009 Foodstuffs - Determination of vitamin B6 by microbiological assay

Proficiency testing schemes 

Here are listed some completed, on-going and/or upcoming proficiency testing schemes concerning vitamin B₆.

DRRR - Deutsches Referenzbüro für Lebensmittel-Ringversuche und Referenzmaterialien

FAPAS

PTA - Proficiency Testing Australia: Report No. 651 Foor Proficiency Testing Program (Round 31) - Vitamins

Livsmedelsverket, National Food Agency of Sweden - Report 24 (2011) Vitamins in Foods - Round V-9

 Some upcoming proficiency testing schemes can be found in the EPTIS database.

Other methods available

• AACC 86-31.01 Vitamin B₆ Complex---Microbiological Method
• AOAC 961.15 Vitamin B6 (Pyridoxine, Pyridoxal, Pyridoxamine) in Food Extracts
  • microbiological
    
• AOAC 985.32 Vitamin B6 (Pyridoxine, Pyridoxal, Pyridoxamine) in Ready-To-Feed Milk-Based Infant Formula
  • microbiological
• EN 14663:2005 Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC

 Literature

•  see separate child page below

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