Difference between revisions of "Summary - B2"

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<p><strong>EN 14152:2003 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Food stuffs &ndash; Determination of vitamin B2 by HPLC:</strong>
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<p><strong>Scope</strong>
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<p>This European Standard specifies a method for the determination of vitamin B2&nbsp;in foodstuffs by high performance liquid chromatography (HPLC). The determination of vitamin B2 content is carried out by measurement of riboflavin.
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<p><strong>Principle</strong>
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<p>Riboflavin in an appropriate sample solution is determined after acid hydrolysis followed by dephosphorylation using an enzymatic treatment by highs performance liquid chromatographic (HPLC) separation with a fluorometric detection.
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<p><strong>Precision data</strong>
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<table>
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<tr>
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<th>
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<p>&nbsp;</p></th>
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<th>
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<p> CRM 421 <br class="atl-forced-newline" />
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Milk powder </p></th>
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<th>
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<p> CRM 487 <br class="atl-forced-newline" />
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Pig liver </p></th>
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</tr>
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<tr>
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<td>
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<p> Repeatability standard deviation s<sub>r</sub>, mg/100 g </p></td>
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<td>
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<p> 0,046 </p></td>
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<td>
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<p> 0,181 </p></td>
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</tr>
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<tr>
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<td>
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<p> Repeatability coefficient of variation, % </p></td>
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<td>
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<p> 3,2 </p></td>
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<td>
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<p> 1,7 </p></td>
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</tr>
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<tr>
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<td>
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<p> Repeatability value r = [2,83 x s<sub>r</sub>], mg/100 g </p></td>
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<td>
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<p> 0,130 </p></td>
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<td>
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<p> 0,511 </p></td>
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</tr>
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<tr>
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<td>
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<p> Reproducibility standard deviation s<sub>R</sub>, mg/100 g </p></td>
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<td>
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<p> 0,106 </p></td>
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<td>
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<p> 0,832 </p></td>
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</tr>
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<tr>
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<td>
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<p> Reproducibility&nbsp;coefficient of variation, % </p></td>
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<td>
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<p> 7,3 </p></td>
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<td>
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<p> 7,9 </p></td>
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</tr>
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<tr>
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<td>
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<p> Reproducibility&nbsp;value r = [2,83 x s<sub>R</sub>], mg/100 g </p></td>
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<td>
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<p> 0,30 </p></td>
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<td>
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<p> 2,35 </p></td>
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</tr></table>
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<p><br class="atl-forced-newline" /></p>
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<p>Data derived from EN 14152:2003.</p>

Revision as of 08:46, 24 May 2017

EN 14152:2003                 Food stuffs – Determination of vitamin B2 by HPLC:


Scope


This European Standard specifies a method for the determination of vitamin B2 in foodstuffs by high performance liquid chromatography (HPLC). The determination of vitamin B2 content is carried out by measurement of riboflavin.


Principle


Riboflavin in an appropriate sample solution is determined after acid hydrolysis followed by dephosphorylation using an enzymatic treatment by highs performance liquid chromatographic (HPLC) separation with a fluorometric detection.


Precision data

 

CRM 421
Milk powder

CRM 487
Pig liver

Repeatability standard deviation sr, mg/100 g

0,046

0,181

Repeatability coefficient of variation, %

3,2

1,7

Repeatability value r = [2,83 x sr], mg/100 g

0,130

0,511

Reproducibility standard deviation sR, mg/100 g

0,106

0,832

Reproducibility coefficient of variation, %

7,3

7,9

Reproducibility value r = [2,83 x sR], mg/100 g

0,30

2,35




Data derived from EN 14152:2003.

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