Difference between revisions of "Summary - β-carotene"
(Created page with " <h4>EN 12823-2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene</h4> <p><strong>Scope</strong>...") |
m (3 revisions imported) |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 20: | Line 20: | ||
<p><strong>Presicion data from EN 12823-2:</strong></p> | <p><strong>Presicion data from EN 12823-2:</strong></p> | ||
| − | <table> | + | <table style="border: 1px solid black;border-collapse: collapse;"> |
| − | <tr> | + | <tr style="border: 1px solid black;border-collapse: collapse;"> |
| + | <th style="border: 1px solid black;border-collapse: collapse;"> | ||
| + | <p> </p> | ||
| − | <th> | + | </th> |
| − | <p> | + | <th style="border: 1px solid black;border-collapse: collapse;"> |
| + | <p> Margarine | ||
| + | <br> </p> | ||
| − | <th> | + | </th> |
| − | < | + | <th style="border: 1px solid black;border-collapse: collapse;"> |
| + | <p> Vitamin drink | ||
| + | <br> </p> | ||
| − | <th> | + | </th> |
| − | < | + | <th style="border: 1px solid black;border-collapse: collapse;"> |
| + | <p> Pudding powder | ||
| + | <br> </p> | ||
| − | <th> | + | </th> |
| − | < | + | <th style="border: 1px solid black;border-collapse: collapse;"> |
| + | <p> Mixed vegetables | ||
| + | <br> </p> | ||
| − | + | </th> | |
| − | + | </tr> | |
| − | </tr> | ||
| − | <tr> | + | <tr style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <td style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <td> | + | <p> Repeatability standard deviation s |
| − | <p> Repeatability standard deviation s<sub>r</sub>, mg/100 g </p></td> | + | <sub>r</sub>, mg/100 g </p> |
| − | + | </td> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> 0,011 <br | + | <p> 0,011 |
| − | + | <br> </p> | |
| − | <td> | + | </td> |
| − | <p> 0,065 <br | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 0,065 | |
| − | <td> | + | <br> </p> |
| − | <p> 0,085 <br | + | </td> |
| − | + | <td style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <td> | + | <p> 0,085 |
| − | <p> 0,71 <br | + | <br> </p> |
| − | </tr> | + | </td> |
| − | + | <td style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <tr> | + | <p> 0,71 |
| − | + | <br> </p> | |
| − | <td> | + | </td> |
| − | <p> Repeatability coefficient of variation, % </p></td> | + | </tr> |
| − | + | <tr style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> 4,5 <br | + | <p> Repeatability coefficient of variation, % </p> |
| − | + | </td> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> 2,9 <br | + | <p> 4,5 |
| − | + | <br> </p> | |
| − | <td> | + | </td> |
| − | <p> 5,6 <br | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 2,9 | |
| − | <td> | + | <br> </p> |
| − | <p> 3,9 <br | + | </td> |
| − | </tr> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 5,6 | |
| − | <tr> | + | <br> </p> |
| − | + | </td> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> Repeatability value r = [2,83 x s<sub>r</sub>], mg/100 g </p></td> | + | <p> 3,9 |
| − | + | <br> </p> | |
| − | <td> | + | </td> |
| − | <p> 0,032 <br | + | </tr> |
| − | + | <tr style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> 0,19 <br | + | <p> Repeatability value r = [2,83 x s |
| − | + | <sub>r</sub>], mg/100 g </p> | |
| − | <td> | + | </td> |
| − | <p> 0,24 <br | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 0,032 | |
| − | <td> | + | <br> </p> |
| − | <p> 2,0 <br | + | </td> |
| − | </tr> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 0,19 | |
| − | <tr> | + | <br> </p> |
| − | + | </td> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> Reproducibility standard deviation s<sub>R</sub>, mg/100 g </p></td> | + | <p> 0,24 |
| − | + | <br> </p> | |
| − | <td> | + | </td> |
| − | <p> 0,024 <br | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 2,0 | |
| − | <td> | + | <br> </p> |
| − | <p> 0,15 <br | + | </td> |
| − | + | </tr> | |
| − | <td> | + | <tr style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> 0,14 <br | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> Reproducibility standard deviation s | |
| − | <td> | + | <sub>R</sub>, mg/100 g </p> |
| − | <p> 2,7 <br | + | </td> |
| − | </tr> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 0,024 | |
| − | <tr> | + | <br> </p> |
| − | + | </td> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> Reproducibility coefficient of variation, % </p></td> | + | <p> 0,15 |
| − | + | <br> </p> | |
| − | <td> | + | </td> |
| − | <p> 9,7 <br | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 0,14 | |
| − | <td> | + | <br> </p> |
| − | <p> 6,5 <br | + | </td> |
| − | + | <td style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <td> | + | <p> 2,7 |
| − | <p> 9,3 <br | + | <br> </p> |
| − | + | </td> | |
| − | <td> | + | </tr> |
| − | <p> 15 <br | + | <tr style="border: 1px solid black;border-collapse: collapse;"> |
| − | </tr> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> Reproducibility coefficient of variation, % </p> | |
| − | <tr> | + | </td> |
| − | + | <td style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <td> | + | <p> 9,7 |
| − | <p> Reproducibility limit r = [2,8 x s<sub>R</sub>], mg/100 g </p></td> | + | <br> </p> |
| − | + | </td> | |
| − | <td> | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | <p> 0,069 <br | + | <p> 6,5 |
| − | + | <br> </p> | |
| − | <td> | + | </td> |
| − | <p> 0,41 <br | + | <td style="border: 1px solid black;border-collapse: collapse;"> |
| − | + | <p> 9,3 | |
| − | <td> | + | <br> </p> |
| − | <p> 0,40 <br | + | </td> |
| − | + | <td style="border: 1px solid black;border-collapse: collapse;"> | |
| − | <td> | + | <p> 15 |
| − | <p> 7,6 <br | + | <br> </p> |
| − | </tr></table> | + | </td> |
| + | </tr> | ||
| + | <tr style="border: 1px solid black;border-collapse: collapse;"> | ||
| + | <td style="border: 1px solid black;border-collapse: collapse;"> | ||
| + | <p> Reproducibility limit r = [2,8 x s | ||
| + | <sub>R</sub>], mg/100 g </p> | ||
| + | </td> | ||
| + | <td style="border: 1px solid black;border-collapse: collapse;"> | ||
| + | <p> 0,069 | ||
| + | <br> </p> | ||
| + | </td> | ||
| + | <td style="border: 1px solid black;border-collapse: collapse;"> | ||
| + | <p> 0,41 | ||
| + | <br> </p> | ||
| + | </td> | ||
| + | <td style="border: 1px solid black;border-collapse: collapse;"> | ||
| + | <p> 0,40 | ||
| + | <br> </p> | ||
| + | </td> | ||
| + | <td style="border: 1px solid black;border-collapse: collapse;"> | ||
| + | <p> 7,6 | ||
| + | <br> </p> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
Latest revision as of 13:33, 9 May 2019
EN 12823-2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene
Scope
This European Standard specifies a method for the determination of total β-carotene in foodstuffs by high performance liquid chromatography (HPLC).
Principle
Determination of the sum of β-carotene isomers in an appropriate sample solution by HPLC and spectrometric detection in the visible range. The extract obtained after saponification as described in EN 12823-1 may be used for quantification. Identification on the basis of the retention times, and determination by the external standard method using peak areas or peak heights.
Internal standards methods may also be used if the corresponding recovery tests have proven the same behaviour of the internal standard during the analysis as the analyte itself.
Presicion data from EN 12823-2:
|
|
Margarine
|
Vitamin drink
|
Pudding powder
|
Mixed vegetables
|
|---|---|---|---|---|
|
Repeatability standard deviation s r, mg/100 g |
0,011
|
0,065
|
0,085
|
0,71
|
|
Repeatability coefficient of variation, % |
4,5
|
2,9
|
5,6
|
3,9
|
|
Repeatability value r = [2,83 x s r], mg/100 g |
0,032
|
0,19
|
0,24
|
2,0
|
|
Reproducibility standard deviation s R, mg/100 g |
0,024
|
0,15
|
0,14
|
2,7
|
|
Reproducibility coefficient of variation, % |
9,7
|
6,5
|
9,3
|
15
|
|
Reproducibility limit r = [2,8 x s R], mg/100 g |
0,069
|
0,41
|
0,40
|
7,6
|
