Difference between revisions of "Summary - vitamin C"
Jump to navigation
Jump to search
EN 14130:2003 Foodstuffs - Determination of vitamin C by HPLC
m (Protected "Summary - vitamin C" ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))) |
m (2 revisions imported) |
(No difference)
| |
Latest revision as of 13:33, 9 May 2019
EN 14130:2003 Foodstuffs - Determination of vitamin C by HPLC
Scope
This European Standard specifies an HPLC-method for the determination of vitamin C in foodstuffs. Vitamin C is the sum of L ( + ) ascorbic acid and dehydro L ( + ) ascorbic acid.
Principle
Vitamin C is extracted from the sample to be analysed using metaphosphoric acid solution. A reducing solution is used to transform dehydro L ( + ) ascorbic acid to L ( + ) ascorbic acid. Total L (+) ascorbic acid content is determined by HPLC with a UV detection at 265 nm.
Precision data
