Difference between revisions of "Summary - B2"

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<p><strong>EN 14152:2003 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Food stuffs &ndash; Determination of vitamin B2 by HPLC:</strong>
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<br class="atl-forced-newline" /></p>
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<p><strong>Scope</strong>
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<br class="atl-forced-newline" /></p>
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<p>This European Standard specifies a method for the determination of vitamin B2&nbsp;in foodstuffs by high performance liquid chromatography (HPLC). The determination of vitamin B2 content is carried out by measurement of riboflavin.
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<br class="atl-forced-newline" /></p>
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<p><strong>Principle</strong>
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<p>Riboflavin in an appropriate sample solution is determined after acid hydrolysis followed by dephosphorylation using an enzymatic treatment by highs performance liquid chromatographic (HPLC) separation with a fluorometric detection.
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<br class="atl-forced-newline" /></p>
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<p><strong>Precision data</strong>
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<br class="atl-forced-newline" /></p>
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<table style="border: 1px solid black;border-collapse: collapse;">
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        <tr style="border: 1px solid black;border-collapse: collapse;" class="tablesorter-headerRow">
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            <th style="border: 1px solid black;border-collapse: collapse;">
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                    <p>&nbsp;</p>
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            </th>
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            <th style="border: 1px solid black;border-collapse: collapse;">
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                    <p> CRM 421
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                        <br> Milk powder </p>
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            </th>
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            <th style="border: 1px solid black;border-collapse: collapse;">
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                    <p> CRM 487
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                        <br> Pig liver </p>
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            </th>
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        </tr>
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        <tr style="border: 1px solid black;border-collapse: collapse;">
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> Repeatability standard deviation s
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                    <sub>r</sub>, mg/100 g </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 0,046 </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 0,181 </p>
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            </td>
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        </tr>
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        <tr style="border: 1px solid black;border-collapse: collapse;">
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> Repeatability coefficient of variation, % </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 3,2 </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 1,7 </p>
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            </td>
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        </tr>
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        <tr style="border: 1px solid black;border-collapse: collapse;">
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> Repeatability value r = [2,83 x s
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                    <sub>r</sub>], mg/100 g </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 0,130 </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 0,511 </p>
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            </td>
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        </tr>
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        <tr style="border: 1px solid black;border-collapse: collapse;">
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> Reproducibility standard deviation s
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                    <sub>R</sub>, mg/100 g </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 0,106 </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 0,832 </p>
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            </td>
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        </tr>
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        <tr style="border: 1px solid black;border-collapse: collapse;">
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> Reproducibility&nbsp;coefficient of variation, % </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 7,3 </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 7,9 </p>
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            </td>
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        </tr>
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        <tr style="border: 1px solid black;border-collapse: collapse;">
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> Reproducibility&nbsp;value r = [2,83 x s
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                    <sub>R</sub>], mg/100 g </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 0,30 </p>
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            </td>
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            <td style="border: 1px solid black;border-collapse: collapse;">
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                <p> 2,35 </p>
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            </td>
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        </tr>
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</table>
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<p><br class="atl-forced-newline" /></p>
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<p>Data derived from EN 14152:2003.</p>

Latest revision as of 13:33, 9 May 2019

EN 14152:2003                 Food stuffs – Determination of vitamin B2 by HPLC:

Scope

This European Standard specifies a method for the determination of vitamin B2 in foodstuffs by high performance liquid chromatography (HPLC). The determination of vitamin B2 content is carried out by measurement of riboflavin.

Principle

Riboflavin in an appropriate sample solution is determined after acid hydrolysis followed by dephosphorylation using an enzymatic treatment by highs performance liquid chromatographic (HPLC) separation with a fluorometric detection.

Precision data

 

CRM 421
Milk powder

CRM 487
Pig liver

Repeatability standard deviation s r, mg/100 g

0,046

0,181

Repeatability coefficient of variation, %

3,2

1,7

Repeatability value r = [2,83 x s r], mg/100 g

0,130

0,511

Reproducibility standard deviation s R, mg/100 g

0,106

0,832

Reproducibility coefficient of variation, %

7,3

7,9

Reproducibility value r = [2,83 x s R], mg/100 g

0,30

2,35




Data derived from EN 14152:2003.

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