Analytical methods - BPA

NPR-CEN/TS 13130-13:2005 Materialen en artikelen in contact met voedingsmiddelen - Kunststofbestanddelen die aan limietwaarde worden onderworpen - Deel 13: Bepaling van 2,2-bis(4-hydroxyphenyl) propaan (Bisphenol A) in namaakvoedingsmiddelen[file:///Y:/Unit_SCT/GOAL/12%20Projects%20-%20GWO/LE005%20-%20FP7-KBBE-2011-2.4.02-TDS_EXPOSURE/03_Project/WP5/Task%205.4.2%20-X-%20Choice%20of%20adequate%20analytical%20methods/wiki%20Organic%20contaminants%20in%20food_info%20wiki.docx#_msocom_1 [JG1]] 

Other methods available

Alternative methods from literature

Analytical methods for the determination of bisphenol A in food (Ballesteros-Gómez 2009) - Review

Determination of bisphenol A (BPA) in these matrices often requires extensive sample preparation prior to instrumental analysis. The typical steps within food sample preparation include pre-treatment, extraction, clean-up, concentration and sometimes derivatization. The solid samples are usually first homogenized while the liquid ones are filtered and/or centrifuged. Special treatments can be required depending on the matrix. Solvent extraction and SPE are the most widely used techniques for the isolation of BPA from solid and liquid samples, respectively, mainly because of its simplicity and wide-range applicability. LC-fluorescence detection is still frequently used for determination of BPA in food and it gives satisfactory quantitative results, but LC-MS and GC-MS are becoming more attractive because they provide more selective and, therefore, more reliable methods.

Chemistry and analytical methods for determination of Bisphenol A in food and biological samples (Cao 2010) - (http://www.who.int/en)

Sensitive and reliable analytical methods are available for the determination of BPA in food. Solvent extraction and SPE are the most commonly used and most effective methods for the extraction of BPA in food . The most reliable method for the detection of BPA  are isotope dilution methods based on MS and MS/MS. Results from non-MS-based methods should be confirmed by MS methods, especially for food samples.

Method of Thomson&Grounds (2005) - Bisphenol A in canned foods in New Zealand: An exposure assessment

 

Canned foods : vegetables, fruit, fish, soup and sauces, canned meat, spaghetti and baked beans, infant foods, beverages

 

Following key steps can be identified  :

Samples less than 1% fat (based on label claims) :

-          Rinse the glasware with ethanol and acetone before use

-          Homogenization of the sample

-          Weigh duplicate 20 g subsamples

-          Store one sample at 4°C

-          Add a accurately known quantity of BPA-d14 (internal standard)

-          Extract the sample with acetonitrile, derivatized with acetic anhydride

-          Measure the samples itrile analysis by gas chromatography/mass spectrometry with selective-ion monitoring.

 

Samples more than 1% fat (based on label claims) :

-          Rinse the glasware with ethanol and acetone before use

-          Homogenization of the sample

-          Weigh duplicate 20 g subsamples

-          Store one sample at 4°C

-          Add a accurately known quantity of BPA-d14 (internal standard)

-          Extract the sample with a (50/50) mixture of acetonitrile (derivatized with acetic anhydride) and trimethylpentane (for fat removal), discare the trimethylpentane layer

-          Measure the extracts by gas chromatography/mass spectrometry with selective-ion monitoring

 

soft drinks :

-          Degass the sample

-          Derivatization of 50 ml sample

-          Analyse the extracts by gas chromatography/mass spectrometry with selective-ion monitoring

 

Method of Ackerman (2010) - Determination of Bisphenol A in U.S. Infant Formulas: Updated methods and concentrations

Food : Infant formula

Following key steps can be identified :

  -          Weigh 5.0 g of the food sample 

-          Add 5 ml of acetonitrile

-          Vortex the sample for 30 s

-          Centrifugation of the sample for 20 min at 4000 rcf

-          Collect the acetonitrile supernatant

-          Dilution of the supernatant with water

-          Loading of the supernatant on a SPE column

-          Elution of the BPA compound with chloroform.

-          Evaporation under nitrogen at 50°C (not dry)

-          Add internal standard, d16-BPA (50 ng)  to each vial

-          Analyse the sample by LC-MS/MS

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 [file:///Y:/Unit_SCT/GOAL/12%20Projects%20-%20GWO/LE005%20-%20FP7-KBBE-2011-2.4.02-TDS_EXPOSURE/03_Project/WP5/Task%205.4.2%20-X-%20Choice%20of%20adequate%20analytical%20methods/wiki%20Organic%20contaminants%20in%20food_info%20wiki.docx#_msoanchor_1 [JG1]]Stef, dit is een norm voor het testen van de bisphenol migratie van verpakking naar voeding - ik zou deze misschien niet als norm nemen omdat we dan niet echt in voeding zitten te meten (eigenlijk kan je deze weg laten)