https://eurofir-wiki.metrofood-services.eu/index.php?action=history&feed=atom&title=Summary_-_biotin 2026-04-15T10:27:12Z Revision history for this page on the wiki MediaWiki 1.32.1 https://eurofir-wiki.metrofood-services.eu/index.php?title=Summary_-_biotin&diff=761&oldid=prev 2019-05-09T13:33:07Z

<p>2 revisions imported</p> <table class="diff diff-contentalign-left" data-mw="interface"> <tr class="diff-title" lang="en-GB"> <td colspan="1" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td> <td colspan="1" style="background-color: #fff; color: #222; text-align: center;">Revision as of 13:33, 9 May 2019</td> </tr><tr><td colspan="2" class="diff-notice" lang="en-GB"><div class="mw-diff-empty">(No difference)</div> </td></tr></table>

Admin https://eurofir-wiki.metrofood-services.eu/index.php?title=Summary_-_biotin&diff=760&oldid=prev 2018-01-08T12:27:20Z

<p>Protected &quot;<a href="/index.php/Summary_-_biotin" title="Summary - biotin">Summary - biotin</a>&quot; ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))</p> <table class="diff diff-contentalign-left" data-mw="interface"> <tr class="diff-title" lang="en-GB"> <td colspan="1" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td> <td colspan="1" style="background-color: #fff; color: #222; text-align: center;">Revision as of 12:27, 8 January 2018</td> </tr><tr><td colspan="2" class="diff-notice" lang="en-GB"><div class="mw-diff-empty">(No difference)</div> </td></tr></table>

Admin https://eurofir-wiki.metrofood-services.eu/index.php?title=Summary_-_biotin&diff=759&oldid=prev 2017-05-12T15:31:16Z

<p>Created page with &quot; ----- ==== EN 15607:2009 Foodstuffs - Determination of d-biotin by HPLC: ==== &#039;&#039;&#039;Scope&#039;&#039;&#039; This European Standard specifies a method for the determination of the mass fract...&quot;</p> <p><b>New page</b></p><div><br /> -----<br /> <br /> ==== EN 15607:2009 Foodstuffs - Determination of d-biotin by HPLC: ====<br /> <br /> '''Scope'''<br /> <br /> This European Standard specifies a method for the determination of the mass fraction of d-biotin by high performance liquid chromatography (HPLC). The method has been validated in an inter-laboratory test on fortified and non-fortified samples such as cereal breakfast powder, infant milk powder, lyophilized green peas with ham, lyophilized chicken soup and on nutritive orange juice, at levels from 16 µg/100 g to 200 µg/100 g.<br /> <br /> NOTE 1 d-biocytin can also be estimated by this method. But none of the samples used for the validation step contained d-biocytin. Nonetheless the recovery rate is more than 90 % for d-biotin and d-biocytin.<br /> <br /> NOTE 2 The method underestimates the real biotin content when used for samples containing egg.<br /> <br /> '''Principle'''<br /> <br /> D-biotin is extracted from food after enzymatic treatment and quantified by HPLC with post-column binding reaction.<br /> <br /> The complexion of d-biotin with avidin appears to be very specific. On that account, this protein, covalently bound to a fluorescent marker, fluorescein 5-isothiocyanate, can be used as a reagent for a post-column derivatisation of d-biotin.<br /> <br /> '''Precision data'''<br /> <br /> * [https://metrofood-wiki.foodcase-services.com/index.php?title=Precision+data+-+biotin link to detailed data]<br /> <br /> <br /> -----<br /> <br />  </div>

Admin