https://eurofir-wiki.metrofood-services.eu/index.php?action=history&feed=atom&title=Summary_-_B6_by_HPLCSummary - B6 by HPLC - Revision history2026-04-15T09:57:16ZRevision history for this page on the wikiMediaWiki 1.32.1https://eurofir-wiki.metrofood-services.eu/index.php?title=Summary_-_B6_by_HPLC&diff=755&oldid=prevAdmin: 3 revisions imported2019-05-09T13:33:07Z<p>3 revisions imported</p>
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</td></tr></table>Adminhttps://eurofir-wiki.metrofood-services.eu/index.php?title=Summary_-_B6_by_HPLC&diff=754&oldid=prevAdmin: Protected "Summary - B6 by HPLC" ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))2018-01-08T12:32:30Z<p>Protected "<a href="/index.php/Summary_-_B6_by_HPLC" title="Summary - B6 by HPLC">Summary - B6 by HPLC</a>" ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))</p>
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</td></tr></table>Adminhttps://eurofir-wiki.metrofood-services.eu/index.php?title=Summary_-_B6_by_HPLC&diff=753&oldid=prevAdmin at 08:41, 23 November 20172017-11-23T08:41:34Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 08:41, 23 November 2017</td>
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<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>'''EN 14164:2008 Foodstuffs <del class="diffchange diffchange-inline">? </del>Determination of vitamin B6 by HPLC:'''</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'''EN 14164:2008 Foodstuffs <ins class="diffchange diffchange-inline">- </ins>Determination of vitamin B6 by HPLC:'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''Scope'''</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''Scope'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>This European Standard specifies a method for the determination of vitamin B<sub>6</sub> in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B<sub>6</sub> is a mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. <del class="diffchange diffchange-inline">The ?</del>-glycosylated forms are not taken into account. These can be determined with the method given in EN14164 by which the different vitamer of vitamin B<sub>6</sub> (pyridoxal, pyridoxamine and pyridoxin) are separated and individually quantified. A third European Standard (EN 14166) determines the total vitamin B<sub>6</sub> by microbiological assay.<br /></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>This European Standard specifies a method for the determination of vitamin B<sub>6</sub> in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B<sub>6</sub> is a mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. <ins class="diffchange diffchange-inline">The β</ins>-glycosylated forms are not taken into account. These can be determined with the method given in EN14164 by which the different vitamer of vitamin B<sub>6</sub> (pyridoxal, pyridoxamine and pyridoxin) are separated and individually quantified. A third European Standard (EN 14166) determines the total vitamin B<sub>6</sub> by microbiological assay.<br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''Principle'''<br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''Principle'''<br /></div></td></tr>
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</table>Adminhttps://eurofir-wiki.metrofood-services.eu/index.php?title=Summary_-_B6_by_HPLC&diff=752&oldid=prevAdmin: Created page with "'''EN 14164:2008 Foodstuffs ? Determination of vitamin B6 by HPLC:''' '''Scope''' This European Standard specifies a method for the determinatio..."2017-05-12T15:31:16Z<p>Created page with "'''EN 14164:2008 Foodstuffs ? Determination of vitamin B6 by HPLC:''' '''Scope''' This European Standard specifies a method for the determinatio..."</p>
<p><b>New page</b></p><div>'''EN 14164:2008 Foodstuffs ? Determination of vitamin B6 by HPLC:'''<br />
<br />
'''Scope'''<br />
<br />
This European Standard specifies a method for the determination of vitamin B<sub>6</sub> in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B<sub>6</sub> is a mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The ?-glycosylated forms are not taken into account. These can be determined with the method given in EN14164 by which the different vitamer of vitamin B<sub>6</sub> (pyridoxal, pyridoxamine and pyridoxin) are separated and individually quantified. A third European Standard (EN 14166) determines the total vitamin B<sub>6</sub> by microbiological assay.<br /><br />
<br />
'''Principle'''<br /><br />
<br />
Pyridoxal, pyridoxamine and pyridoxine are extracted from food by acid hydrolysis and dephosphorylated enzymatically using acid phosphatase.<br /><br />
<br />
By reaction with glyoxylic acid in the presence of Fe<sup>2+</sup> as a catalyst, pyridoxamine is transformed into pyridoxal, which is then reduced to pyridoxine by the action of sodium borohydride in alkaline medium. Pyridoxine is then quantified in the sample solution by HPLC with a fluorometric detection.<br /><br />
<br />
'''Precision data'''<br /><br />
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* [https://metrofood-wiki.foodcase-services.com/index.php?title=Precision+data+-+B6+by+HPLC link to detailed data]<br /></div>Admin