Admin: 3 revisions imported

2019-05-09T13:33:10Z

3 revisions imported

Admin: Protected "Summary - β-carotene" ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

2018-01-08T12:30:24Z

Protected "Summary - β-carotene" ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

Admin at 08:23, 23 November 2017

2017-11-23T08:23:55Z

Show changes

Admin: Created page with "

EN 12823-2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene

Scope..."

2017-05-24T12:17:22Z

Created page with " <h4>EN 12823-2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene</h4> Scope..."

New page

<h4>EN 12823-2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene</h4>

Scope

This European Standard specifies a method for the determination of total β-carotene in foodstuffs by high performance liquid chromatography (HPLC).

Principle

Determination of the sum of β-carotene isomers in an appropriate sample solution by HPLC and spectrometric detection in the visible range. The extract obtained after saponification as described in EN 12823-1 may be used for quantification. Identification on the basis of the retention times, and determination by the external standard method using peak areas or peak heights.

Internal standards methods may also be used if the corresponding recovery tests have proven the same behaviour of the internal standard during the analysis as the analyte itself.

Presicion data from EN 12823-2:

<table>
<tr>

<th>
 </th>

<th>
 Margarine </th>

<th>
 Vitamin drink </th>

<th>
 Pudding powder </th>

<th>
 Mixed vegetables </th>
</tr>

<tr>

<td>
 Repeatability standard deviation sr, mg/100 g </td>

<td>
 0,011 </td>

<td>
 0,065 </td>

<td>
 0,085 </td>

<td>
 0,71 </td>
</tr>

<tr>

<td>
 Repeatability coefficient of variation, % </td>

<td>
 4,5 </td>

<td>
 2,9 </td>

<td>
 5,6 </td>

<td>
 3,9 </td>
</tr>

<tr>

<td>
 Repeatability value r = [2,83 x sr], mg/100 g </td>

<td>
 0,032 </td>

<td>
 0,19 </td>

<td>
 0,24 </td>

<td>
 2,0 </td>
</tr>

<tr>

<td>
 Reproducibility standard deviation sR, mg/100 g </td>

<td>
 0,024 </td>

<td>
 0,15 </td>

<td>
 0,14 </td>

<td>
 2,7 </td>
</tr>

<tr>

<td>
 Reproducibility coefficient of variation, % </td>

<td>
 9,7 </td>

<td>
 6,5 </td>

<td>
 9,3 </td>

<td>
 15 </td>
</tr>

<tr>

<td>
 Reproducibility limit r = [2,8 x sR], mg/100 g </td>

<td>
 0,069 </td>

<td>
 0,41 </td>

<td>
 0,40 </td>

<td>
 7,6 </td>
</tr></table>

← Older revision	Revision as of 13:33, 9 May 2019
(No difference)

← Older revision	Revision as of 12:30, 8 January 2018
(No difference)

Summary - β-carotene - Revision history

Admin: 3 revisions imported

Admin: Protected "Summary - β-carotene" ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

Admin at 08:23, 23 November 2017

Admin: Created page with "

EN 12823-2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene