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Figure 1. Pantothenic acid, C9H17NO5, Mw = 219,23 g/mol The only vitamin active form of pantothenic acid is dextro- form. In foods, pantothenic acid is mo..."

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Created page with " Figure 1. Pantothenic acid, C9H17NO5, Mw = 219,23 g/mol The only vitamin active form of pantothenic acid is dextro- form. In foods, pantothenic acid is mo..."

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Figure 1. Pantothenic acid, C9H17NO5, Mw = 219,23 g/mol

The only vitamin active form of pantothenic acid is dextro- form. In foods, pantothenic acid is most often in a form of salt or bound to proteins.

Synonyms for pantothenic acid:

* vitamin B5, pantothenate, chick antidermatitis factor

 

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=== Golden standard ===

* No EN Standard available.

* [http://www.aoac.org/omarev1/945_74.pdf AOAC 945.74] Pantothenic Acid in Vitamin Preparations
** Microbiological assay described in [http://www.aoac.org/omarev1/960_46.pdf AOAC 960.46.]

=== Method indicator ===

* ''Name''
* ''Code''

=== Scope ===

The method AOAC 945.74 describes a microbiological method for the determination of pantothenic acid.

=== Principle ===

After extraction, pantothenic acid content of the sample is determined by microbiological assay using turbidimetric method.

=== Key steps ===

==== Preparing of the sample test solution ====

* The pH of the solution should be maintained below 7,0.
* Water is added and pH of the sample solution is adjusted to 5,65 with CH3COOH or CH3COONa.
* Solution is autoclaved (5-7 min, 121-123 °C).
* Sample treatments are continued according to AOAC 944.13.

==== Microbiological assay ====

* Microbiological assay is carried out according to AOAC 960.46 using calcium pantothenate standard solution, basal medium stock solution with ingredients that are suitable for determination of pantothenic acid, and inoculum with ''Lactobacillus plantarum'' ATCC 8014.

===== Preparation of test tubes =====

* Microbiological assay is performed using test tubes.
* All of the blanks, standard solutions and samples are prepared at least in duplicates.
* Both an uninoculated and inoculated blank are prepared.
* Test tubes having four different dilution levels of sample solutions are prepared.
* Water and basal medium stock solution are added to test tubes.
** The total volume of the solution in every test tube is 10 ml.
* Test tubes are sterilised prior to inoculation of test tubes.
** Only unincoulated blanks are not inoculated.

===== Determination by turbidimetric method =====

* Tubes are incubated at 30-40 °C for 16-24 h.
* Photometer is calibrated with standard stock solutions.
** Calibration curve is formed by plotting the transmittance (%) for every dilution level against the corresponding content (mg dry weight).
* The test tube solutions are transferred to optical cells and transmittance is measured photometrically.
* Spectrometer is set to 540-660 nm and adjusted to 100 % transmission for uninoculated blanks.
* After achieving a steady state, absorbance of test tubes are read.

==== Calculation ====

* The results obtained are potency of product expressed as D-pantothenic acid.
** The value must be multiplied by 1,087 to express the results as calcium pantothenate and by 1,100 to express the results as sodium pantothenate.
* Pantothenic acid content/ml is calculated.
* Average of values are calculated taking into account such samples that do not vary by more than 10 % from the average.

 

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=== Remarks ===

* The method can be used only for determination of calcium pantothenate or other free forms of pantothenic acid.
* pH of the sample solution should not exceed the value 7,0.

=== Criteria for analytical performance and analytical quality control ===

* Greenfield and Southgate discuss the criteria for analytical performance and quality of analytical data in their book.
** [ftp://ftp.fao.org/docrep/fao/008/y4705e/y4705e00.pdf Greenfield H, Southgate DAT. 2003. Food composition data ? production, management and use. Elsevier Applied Science, London, UK.]

=== Certified Reference Materials/Standard Reference Material ===

* [https://metrofood-wiki.foodcase-services.com/index.php?title=Reference+materials+-+pantothenic+acid link to detailed data]

=== Proficiency testing schemes ===

Here are listed some completed, on-going and/or upcoming proficiency testing schemes concerning pantothenic acid:

[http://www.dla-lvu.de/english/DLA%20Programm%202012%20english.html DLA - Dienstleistung Lebensmittel Analytik GbR: 33/2012 Food Supplement II: Biotin, Vitamin C, Niacin and Pantothenic Acid]

[http://otis.jtlnet.com/~proficie/proficiencytesting.com.au/documents/651.pdf PTA - Proficiency Testing Australia: Report No. 651 Foor Proficiency Testing Program (Round 31) - Vitamins] 

Some upcoming proficiency testing schemes can be found in the [http://www.eptis.bam.de/en/index.htm EPTIS database].

=== Other methods available ===

* AOAC 945.73 Calcium Pantothenate in Vitamin Preparations
** spectrophotometric
* AOAC 992.07 Pantothenic Acid in Milk-Base Infant Formula
** microbiological, turbidimetric

=== Literature ===

* see separate child page below

== EuroFIR assistance to this method/guidelines ==

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  <br /> Figure 1. Pantothenic acid, C9H17NO5, Mw = 219,23 g/mol

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 * Greenfield and Southgate discuss the criteria for analytical performance and quality of analytical data in their book.
-** [ftp://ftp.fao.org/docrep/fao/008/y4705e/y4705e00.pdf Greenfield H, Southgate DAT. 2003. Food composition data ? production, management and use. Elsevier Applied Science, London, UK.]
+** [ftp://ftp.fao.org/docrep/fao/008/y4705e/y4705e00.pdf Greenfield H, Southgate DAT. 2003. Food composition data - production, management and use. Elsevier Applied Science, London, UK.]
 === Certified Reference Materials/Standard Reference Material ===

Pantothenic acid - Revision history

Admin: 4 revisions imported

Admin at 08:40, 9 January 2018

Admin: Protected "Pantothenic acid" ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

Admin at 08:15, 23 November 2017

Admin: Created page with " Figure 1. Pantothenic acid, C9H17NO5, Mw = 219,23 g/mol The only vitamin active form of pantothenic acid is dextro- form. In foods, pantothenic acid is mo..."

Admin: Created page with "

Figure 1. Pantothenic acid, C9H17NO5, Mw = 219,23 g/mol The only vitamin active form of pantothenic acid is dextro- form. In foods, pantothenic acid is mo..."