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Manganese concentration depends on food; therefore, accurate determination requires a sensitive analytical method and freedom from contamination. Inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES) EuroFIR Method Indicators [http://ethesaurus.eurofir.org/ MI1209] are the reliable methods preceded by wet digestion [http://ethesaurus.eurofir.org/ MI1018] or pressure digestion.

Method features are described in [http://www.iso.org/iso/iso_catalogue/catalogue_tc/catalogue_detail.htm?csnumber=36250 ISO 11885:2007] Water quality -- Determination of selected elements by inductively coupled plasma optical emission spectrometry (ICP-OES), [http://www.iso.org/iso/iso_catalogue/catalogue_tc/catalogue_detail.htm?csnumber=32957 ISO 17294:2004]: Water quality -- Application of inductively coupled plasma mass spectrometry (ICP-MS) -- Part 2: Determination of 62 elements,

Other methods are available: [http://www.eoma.aoac.org/ AOAC] ([http://www.eoma.aoac.org/methods/info.asp?ID=29031 AOAC 984.27]) and [http://www.google.pt/url?sa=t&rct=j&q=&esrc=s&frm=1&source=web&cd=1&ved=0CC4QFjAA&url=http%3A%2F%2Fwww.codexalimentarius.net%2Fdownload%2Fstandards%2F388%2FCXS_234e.pdf&ei=FnuZT-eqEYKd0AWMqvDuBQ&usg=AFQjCNEK03RDcLzyEhyhF-lBGh8Cd5cMxQ&sig2=vLRsQ8_-fOV1yBb1_4bZWw CODEX]. [http://books.google.pt/books?id=KQRzKDr9bgcC&printsec=frontcover&hl=pt-PT&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false Southgate and Greenfield Book]

Recently papers were published for calcium determination [http://www.ncbi.nlm.nih.gov/pubmed/22468357 Poitevin et al, 2012]; [http://www.sciencedirect.com/science/article/pii/S0039914002004691 Kmetova, et al 2002].; [http://www.sciencedirect.com/science/article/pii/S0308814605009271 Rennan G.O. Araujo, et al.]; [http://www.sciencedirect.com/science/article/pii/S0889157509000799 Valfredo Azevedo Lemos, et al.]; [http://www.sciencedirect.com/science/article/pii/S0308814696003287 U. Tinggi et al.]

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