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Copper concentration depends on food; therefore, accurate determination requires a sensitive analytical method and freedom from contamination. Inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES) Euro FIR Method Indicators [http://ethesaurus.eurofir.org/ MI1209] are the reliable methods preceded by wet digestion [http://ethesaurus.eurofir.org/ MI1018] or pressure digestion .

Method features are described in [file://insarj/shares/DAN/UR/URMR/EuroFIR%20Nexus/GAMA/ISO%20118852007%20-%20Water%20quality%20--%20Determination%20of%20selected%20elements%20by%20inductively%20coupled%20plasma%20optical%20emission%20spectrometry%20(ICP-OES).mht ISO 11885: 2007] Water quality -- Determination of selected elements by inductively coupled plasma optical emission spectrometry (ICP-OES), [file://insarj/shares/DAN/UR/URMR/EuroFIR%20Nexus/GAMA/ISO%2017294-22003%20-%20Water%20quality%20--%20Application%20of%20inductively%20coupled%20plasma%20mass%20spectrometry%20(ICP-MS)%20--%20Part%202%20Determination%20of%2062%20elements.mht ISO 17294:2003]: Water quality -- Application of inductively coupled plasma mass spectrometry (ICP-MS) -- Part 2: Determination of 62 elements, [http://esearch.cen.eu/esearch/Details.aspx?id=6277905 EN 14084:2003] Foodstuffs - Determination of trace elements - Determination of lead, cadmium, zinc, copper and iron by atomic absorption spectrometry (AAS) after microwave digestion, [http://esearch.cen.eu/esearch/Details.aspx?id=6277173 EN 14082:2003] Foodstuffs - Determination of trace elements - Determination of lead, cadmium, zinc, copper, iron and chromium by atomic absorption spectrometry (AAS) after dry ashing. Other methods are available: [http://www.eoma.aoac.org/ AOAC] and [http://www.google.pt/url?sa=t&rct=j&q=&esrc=s&frm=1&source=web&cd=1&ved=0CC4QFjAA&url=http%3A%2F%2Fwww.codexalimentarius.net%2Fdownload%2Fstandards%2F388%2FCXS_234e.pdf&ei=FnuZT-eqEYKd0AWMqvDuBQ&usg=AFQjCNEK03RDcLzyEhyhF-lBGh8Cd5cMxQ&sig2=vLRsQ8_-fOV1yBb1_4bZWw CODEX].

Recently papers were published for copper determination [http://www.ncbi.nlm.nih.gov/pubmed/22468357 Poitevin,2012], [http://ec.europa.eu/food/fs/sc/scf/out176_en.pdf EFSA, 2003], [http://www.ncbi.nlm.nih.gov/pubmed/18761163 Schiavo D, Neira JY, Nóbrega JA, 2008].

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